Pollution of agricultural origin is considered the most important cause of deterioration of water bodies in our planet. Monitoring studies on pesticides indicate that contamination at source is responsible for between 40 and 90% of the total load of pesticides existing in aquifers. In this context, the main objective of this subproject 2 is to develop the necessary analytical methodologies for, in collaboration with the subproject 1, evaluate various strategies for degradation of these compounds with different organisms and in different real situations. The next large work-package within this sub-project will focus on the study of the degradation routes of pesticides by ligninolytic fungi. To this end, in a first stage, various pesticides representative of different chemical classes will be selected and investigated, and their possible transformation products (TPs) identified with the help of high resolution (Orbitrap) mass spectrometry techniques. The subsequent step will be to assess the degradation of pesticides in real effluents. Finally, the last work-package of this subproject will focus on the study of the capacity of degradation of pesticides of other microorganisms, in particular, microalgae and cyanobacteria.